Syzygium sp (Myrtaceae) Extracts: Inhibition of Alpha Amylase.

ABSTRACT

Aims: The aims of this study were a) prepare crude extracts of commercial teas Syzygium sp by different methods (maceration, decoction and infusion) in different proportions (120, 150 and 1100) and b) submit them the inhibition assays digestive enzyme alpha-amylase before and after exposure to a simulated gastric fluid. Place and Duration of Study Laboratório de Bioquímica, Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Brazil, between August 2012 and July 2013. Methodology: Five samples of Syzygium sp teas were purchased commercially from pharmacies, drugstores and health food stores. Results: In the first step - inhibition of enzymatic extracts of the teas without simulated gastric fluid - the results showed significant inhibition of alpha-amylase (mean 92.84%, CV = 3.86%). However, when these extracts were placed in contact with the simulated gastric fluid was observed decreased inhibition of alpha – amylase, suggesting a possible reduction of hypoglycemic potential of these teas under physiological conditions. Conclusion: Aqueous extract Syzygium sp have high percentages of inhibition of the enzyme amylase, suggesting a potential hypoglycemic effect. Additional studies aiming at elucidating the chemical compounds present in the extracts of Syzygium sp using chromatographic techniques will be performed.