Influence of Hyperferric and Hypoferric Diets on Iron Transporters Proteins in the Rat Mammary Glands.
Article
in English
| IMSEAR
| ID: sea-164585
ABSTRACT
Objectives:
This study aimed to assess the expression of transferrin, lactoferrin and ferroportin at mammary glands of rats after treatment with a diet containing high iron dose and another iron-deficient.Methods:
Female rats have received commercial food, formulated with different iron concentrations, from 2 weeks before mating to 15º day after delivery Hyperferric – with 1,500 ppm of iron (n = 5); Control – with 35 ppm of iron (n = 5); Hypoferric – with 1 ppm of iron (n = 5). At the 15º Day after delivery, mammary glands were extracted and the expression of transferrin, lactoferrin and ferroportin was evaluated by western blotting technique.Results:
Considering control-treatment results as being 100% pattern of the protein expression, the hiperferric diet has increased 17.82% the mean of transferrin expression, 27.50% of the lactoferrin and 63.79% of the ferroportin. The hipoferric diet has increased 13.33% the mean of transferrin expression, 44.59% of the lactoferrin and 20, 50% of the ferroportin.Conclusions:
Both stimuli, hiperferric and hipoferric diet, have increased the expression of the 3 proteins related with iron transport. Under influence of hyperferric diet, remarkable increased was observed for ferroportin, protein related to iron exportation from the cell. Under influence of hypoferric diet a significant increased was observed for lactoferrin, one of the major iron-binding protein in milk.
Full text:
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Index:
IMSEAR (South-East Asia)
Language:
English
Year:
2015
Type:
Article
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