Analgesic and anti-infl ammatory properties of the aqueous extract leaves of Murraya koenigii linn in small animal models.

ABSTRACT

Background: The present study was carried out to evaluate the analgesic activity of aqueous extract leaves of Murraya koenigii linn in Albino rats using tail fl ick method, Eddy’s hot plate methods and anti-infl ammatory activity in Carrageenan induced paw edema in rats. Methods: The analgesic activity was evaluated using Eddy’s hot plate induced hyperalgesia and tail fl ick method, which served as thermal induced pain, where the animal were placed on the hot plate and the reaction time to (lick the paw/ jump out) from the hot plate was observed, 0, 30, 60, 90 mins. Murraya 300 mg, 600 mg/kg/body weight (BW) and ibuprofen (5 mg/kg BW) was administered per oral. The anti-infl ammatory activity was measured by Carrageenan induced paw edema volumes at 0, 1, 2, 3 and 4 hrs using mercury plethysmometer, which served as chemical induced pain models. Results: The mean reaction time in Murraya at a dose of 600 mg/kg at 0 min 5.45±0.72, at 30 mins 6.52±1.03, at 60 mins 7.6±0.81, at 90 mins 8.8±0.63 respectively. The mean reaction time increased signifi cantly with Murraya at dose of 600 mg/kg when compared with control. In the ibuprofen group, the mean reaction time at 0 hr was 0.28±0.04, at 1 hr 0.34±0.05, at 2 hrs 0.46±0.03, at 3 hrs 0.61±0.05, at 4 hrs 0.76±0.05. The mean reaction time Murraya in group 600 mg/kg at 0 hr 0.27±0.04, at 1 hrs 0.39±0.03, at 2 hrs 0.48±0.06, at 3 hrs 0.68±0.05, at 6 hrs 0.80±0.03, respectively. Conclusions: The results indicate that the aqueous extract of Murraya (leaf) extract revealed signifi cant analgesic and anti-infl ammatory in thermal and chemical induced pain models.