Potential of commercial anti-D reagents in the identification of partial D variants in Indian population.
Article
in English
| IMSEAR
| ID: sea-16599
ABSTRACT
BACKGROUND & OBJECTIVE:
As partial D variants are of clinical importance in transfusion medicine, the present study aims to determine the efficiency of commercial anti-D reagents to identify partial D variants.METHODS:
Forty two samples of known partial D identified in the Indian population were tested with seven commercial monoclonal anti-D reagents.RESULTS:
Most of the monoclonal anti-D reagents gave strong positive reactions (24 to 59%) to weak positive (28 to 47%) with partial D cells. Polyclonal anti-D detected all partial D variants as RhD positive, though reacting weakly with the majority (83%) of them. All the seven commercial monoclonal anti-D reagents detected some variants as D negative. Analysis of pairs of these reagents showed that the combinations of reagents 1 & 2 and 1 & 6 could detect all partial D variants as RhD positive and hence can be used for donor testing. INTERPRETATION &CONCLUSION:
Findings of our study showed that none of the monoclonal reagents when used individually could detect all partial D variants. A combination of two suitable anti-D reagents are necessary to detect maximum number of partial D variants.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rh-Hr Blood-Group System
/
Humans
/
Blood Grouping and Crossmatching
/
Indicators and Reagents
/
Isoantibodies
Type of study:
Prognostic study
Language:
English
Year:
2007
Type:
Article
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