Evaluation of In Vitro Antioxidant and Antimicrobial Activity of Mentha Spicata L. Leaves.

ABSTRACT

Different extracts of Mentha spicata L. leaves were investigated for their in vitro antioxidant and antimicrobial activities. In vitro antioxidant activity was evaluated for chloroform, ethanol and aqueous extracts for scavenging of DPPH, Superoxide and FRAP radicals. Antimicrobial activity of the extracts was evaluated against five selected bacterial strains by using cylinder plate assay. Concentration dependent antioxidant activity was observed for all the tested extracts. All the tested extracts of Mentha spicata showed considerable zone of inhibition against the tested bacterial strains. All the extracts showed significant zone of inhibition at a dose of 500μg/ml. Of the three extracts tested the highest zone of inhibition was shown by ethanolic extract against B.subtilis at the dose of 500μg/ml. All extracts exhibited antioxidant and antimicrobial activity in a dose dependent manner.