An immunoradiometric assay for measurement of serum thyroglobulin.
Article
in English
| IMSEAR
| ID: sea-16826
ABSTRACT
A two-site immunoradiometric assay (IRMA) for estimation of serum thyroglobulin (tg) is optimized using partially purified rabbit anti-tg antibodies (ammonium sulphate precipitated) as a solid phase antibody and affinity purified 125I labeled anti-tg antibodies as a tracer. The IRMA correlated well (r = 0.93, n = 200, P < 0.001) with conventional radioimmunoassay (RIA). The inter- and intra-assay coefficient of variation were comparable for both methods. The IRMA method gave a superior sensitivity and working range (2 ng/ml, 3-1000 ng/ml) as compared to that of RIA method (6 ng/ml, 9-500 ng/ml). Using IRMA the total incubation time was curtailed to 4 h as compared to 90 h required for conventional RIA. The shelf-life of tracer antibody used in IRMA was much longer (3 months) as compared to tracer tg (3-4 wk) used in RIA. The optimized IRMA for quantitation of serum tg has superior assay characteristics as compared to RIA.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Thyroglobulin
/
Humans
/
Immunoradiometric Assay
/
Sensitivity and Specificity
Type of study:
Diagnostic study
Language:
English
Year:
1992
Type:
Article
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