Biophysicochemical Evaluation and Micropropagation Studies on Neem for Biodiesel Production.

ABSTRACT

In this experiment the seed oils of 30 Neem (Azadirachta indica. A. juss) biotypes were screened and evaluated for their physio-chemical parameters for oil content, biodiesel yield, density, viscosity, iodine value , free fatty acid, saponification value, flash point and fire point which were estimated for selection of the elite neem biotype. The best shoot regeneration (60%-80%) was observed in Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid NAA (0.2-0.4 mg/L) and benzyl amino purine BAP (0.2-0.4 mg/L). Root induction (80%) was successfully obtained in MS medium supplemented with IBA (0.05 mg/L) and IAA (0.05 mg/L). Acclimatization and hardening was quite successful with survival rate of 60%.