Adsorption Kinetics Studies of Endoglucanase from Rhizopus Oryzae on Activated Charcoal.

ABSTRACT

The adsorption kinetics and activities of endoglucanase enzyme from Rhizopus oryzae were evaluated using activated commercial charcoal as adsorbent. The effect of various experimental parameters on adsorption of endoglucanase such as initial enzyme concentration, amount of adsorbent, contact time and temperature were investigated. The pseudo-first-order and pseudo second-order kinetic models were used to describe the kinetic data which showed that the adsorption of the enzyme followed the pseudo-first order rate expression. The adsorbed enzyme was subjected to saccharification in presence of commercially available carboxy methyl cellulose and the reusability of the adsorbed enzyme was also tested.