Improved RP-HPLC Method for Quantitation of Swertiamarin from Swertia Densifolia Leaves and Marketed Herbal Formulations.
Article
in English
| IMSEAR
| ID: sea-168656
ABSTRACT
Objective:
Present study aims at developing and validating an improved RP-HPLC method for determination of Swertiamarin from extracts of Swertia densifolia (Griseb.) Kashyap leaves and marketed herbal formulations.Methods:
Developed RP-HPLC method is validated and is used for determination of Swertiamarin from hydroalcoholic extracts of test plant material and marketed herbal formulations. The separation is carried out on C18 column. The mobile phase used is 10 mM Ammonium acetate Acetonitrile Formic acid in ratio 90100.2% (v/v/v/). The wavelength selected is 237 nm using UV detector. Results andDiscussion:
A seven-point calibration curve over the concentration range of 50.00 –20000.00 ng/mL for Swertiamarin provides an optimum linear detector response (with r2>0.9970). The % R.S.D. (n = 6) for the low, middle and high quality control samples are within <6.44 and < 9.04 for inter-day and intra-day precision and accuracy respectively.Conclusion:
A simple, sensitive and precise method is developed for quantitation of SWM. Method is validated as per International Conference on Harmonization (ICH) guidelines and its applicability is demonstrated by successful measurement of Swertiamarin from hydroalcoholic extracts of Swertia densifolia leaves and selected marketed herbal formulations.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Type of study:
Qualitative research
Language:
English
Year:
2015
Type:
Article
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