Plasma Epstein-Barr Virus (EBV) DNA as a Biomarker for EBV-associated Hodgkin lymphoma.
Indian Pediatr
;
2015 Aug; 52(8): 681-685
Article
in English
| IMSEAR
| ID: sea-171840
ABSTRACT
Objective:
To assess plasma Epstein-Barr virus (EBV) DNA as a biomarker of tumour burden at diagnosis and during therapy in children with Hodgkin lymphoma.Design:
Case-control study, with prospective follow-up of the Hodgkin lymphoma cohort (2007-2012).Setting:
Pediatric Hematology Oncology unit of a tertiary care hospital in Delhi. Patients Thirty children with Hodgkin lymphoma and 70 sex and age-matched controls (benign lymphadenopathy 19, non-lymphoid malignancy 29, Burkitt lymphoma 5, healthy children 17). Intervention Positive EBV-staining on immunohistochemistry was defined as EBV-associated Hodgkin lymphoma. Plasma EBV real-time quantitative polymerase chain reaction (PCR) was tested at presentation, after first and last chemotherapy cycles, and on follow-up. Main outcomemeasures:
Plasma EBV quantitative PCR was compared between cases and controls. Its kinetics was assessed during and after chemotherapy.Results:
EBV quantitative PCR was positive in 19 (63%) Hodgkin lymphoma cases (range 500–430,000 copies/mL), with 87.5% accuracy (kappa=0.69) as compared with EBVimmunohistochemistry. Sensitivity and specificity of the quantitative PCR were 87.5% and 81.8%, respectively. Only boys showed positive EBV immunohistochemistry and/or quantitative- PCR positivity. All controls were quantitative-PCR negative. All quantitative-PCR positive cases with follow-up blood sample showed EBV clearance after the first cycle. A quantitative-PCR negative case in long-term remission became positive at relapse. EBV status did not influence survival.Conclusion:
Plasma EBV-DNA, detectable in EBV-associated Hodgkin lymphoma, becomes undetectable early after initiating therapy. It can be used as a biomarker of treatment response in EBV-associated Hodgkin lymphoma.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Type of study:
Observational study
/
Risk factors
Language:
English
Journal:
Indian Pediatr
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS