Ferulic Acid Reduces Cell Viability through Its Apoptotic Efficacy: An In vitro Approach.
Br J Med Med Res
;
2015; 5(5): 612-621
Article
in English
| IMSEAR
| ID: sea-175922
ABSTRACT
Aim:
Ferulic acid, a well known dietary phenolic antioxidant, possesses diverse pharmacological and biochemical effects, including anti-inflammatory, hepatoprotective, antidiabetic and anticancer properties. The present study explores the cytotoxic potential of ferulic acid using Hep-2 cell line by analyzing its effect on cell viability, reactive oxygen species generation, apoptotic induction, nuclear damage, DNA fragmentation and expression of apoptosis related proteins. Materials andMethods:
The effect of ferulic acid (2.5, 5, 10, 20 and 40 μg/ml) on Hep-2 cells viability for 24 hr was determined by MTT assay. To substantiate the cytotoxic effect of ferulic acid, the intracellular ROS level was determined using DCFH-DA assay; apoptosis by dual staining; nuclear damage by DAPI staining; DNA fragmentation by using agarose gel electrophoresis; apoptosis related proteins by western blotting.Results:
Ferulic acid significantly inhibited the Hep-2 cell growth in a dose dependent manner and ferulic acid treated Hep-2 cells exhibited features of apoptosis and increase in nuclear damage and DNA fragmentation. We also observed excess reactive oxygen species generation in ferulic acid treated Hep-2 cells. Apoptosis related proteins (p53, Bcl-2, Bax, Caspase 3 & Caspase 9) were significantly modulated in favour of programmed cell death in ferulic acid treated cells.Conclusion:
We thus conclude that the cytotoxic potential of ferulic acid might be due to its role in apoptosis induction, excessive ROS generation and DNA fragmentation in Hep-2 cells.
Full text:
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Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
Br J Med Med Res
Year:
2015
Type:
Article
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