Fetal Liver CD34+-Cells in Dynamic of Human Fetus Development.

ABSTRACT

Fetal liver stem cells are not homogeneous but represented mostly by polyploidy hepatocytes and hepatoblasts and likewise early hematopoietic cells. Both cells lately used in regenerative medicine. However, fact remains unclear that, in which period of gestation preferably produces liver parenchyma stem cells and hematopoietic stem cells. In this case practically there is absence of phenotypic characteristics of liver cells in the dynamics of its development. Phenotype of cells reflects the status of its genome, therefore such information are necessary for further development of cell transplantation. Aim: To create CD34+ phenotypic map of fetal stem cells, isolated from abortive material (fetal liver) obtained by means of MTP in gestation periods from 6 to 20 weeks. Methods: Flow cytometry phenotypic characteristics of hematopoietic fetal stem cells CD34+-cells. Results: Results show that maximum total amount of fetal liver cells could be isolated in the gestation period 20 weeks, minimum - in the gestation period of 6 to 7 weeks. Maximum absolute quantity of CD34+-cells observed in the period of gestation 20 weeks, minimum - in the period of gestation 6 to 7 weeks. Viability of the cells while using our method of isolation is high and varies from 91.5 to 95.5%. The highest percent of CD34+-cells observed in the gestation period 11 weeks, and lowest - in the period of gestation 6 to 7 weeks. Conclusion: Obtaining results allows making preliminary conclusion, that optimal period for isolation of fetal liver hematopoietic CD34+-cells are 11 weeks and 18 to 20 weeks periods of gestation age.