Isolation, Identification and Antimicrobial Susceptibility pattern of Pseudomonas Aeruginosa from Various Clinical Specimens at a North India Hospital.

ABSTRACT

Background: Pseudomonas aeruginosa (P. aeruginosa) is a gram negative bacilli. It is an opportunistic human pathogen and plays an important role in nosocomial infection. It is hard to treat because some factor and several mechanisms are involved in resistant organism. Aim: Isolation, Identification and Antimicrobial susceptibility pattern of Pseudomonas aeruginosa. Methods: This study was conducted during October 2014 to September 2015.Total 2492 samples were collected in which 822 samples show growth. Out of 822, 68 samples were positive for Pseudomonas aeruginosa on the basis of their growth on culture media, oxidase test and biochemical tests. The Antimicrobial susceptibility test of isolates was performed by Kirby-Bauer disc diffusion method according to CLSI guidelines (2014). Results: Majority of Pseudomonas aeruginosa was isolated from pus, urine and swab. The isolated pathogens were maximum sensitivity to Imepenem (88.24%) followed by Meropenem (83.82%), Piperacillin-Tazobactam (82.35%) and were maximum resistance to Gentamicin (61.76%), Tobramycin (60.29%).Conclusion: To conclude, Imepenem, Meropenem and Piperacillin-Tazobactam were found to be the most effective antimicrobial drugs. It should be used in limit. The use of Gentamicin and Tobramycin should be reduced.