Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus.
Article
in English
| IMSEAR
| ID: sea-17873
ABSTRACT
BACKGROUND & OBJECTIVES:
Group A Streptococcus, causative agent of several clinical manifestations codes for multiple protein invasins which help the bacterium to enter non-phagocytic cells. C5a peptidase (SCPA) is a surface protein conserved among different serotypes of M1 strain. The present study was taken up to study SCPA promoted fibronectin independent entry of GAS into epithelial cells.METHODS:
An isogenic 90226 emm1deltaAB (M1(-)) mutant was constructed with thermosensitive pGhost vector. This isogenic M1(-) mutant expressed SCPA on the surface as determined by Western blotting and immunofluorescence.RESULTS:
On preincubation with anti-SCPA serum, the isogenic M1(-) strain exhibited 54 per cent decreased invasion as compared to the bacteria incubated with control serum. Also, purified recombinant SCPA proteins blocked internalization of M1(-) streptococci into HEp-2 cells. The M1(-) strain invaded at the same efficiency in the presence or absence of fibronectin. INTERPRETATION &CONCLUSION:
These results suggested that SCPA acted as a potential invasin of group A streptococcus and promoted invasion independent of fibronectin.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Endopeptidases
/
Streptococcus pyogenes
/
Humans
/
Cell Line
/
Blotting, Western
/
Fibronectins
/
Adhesins, Bacterial
/
Epithelial Cells
Language:
English
Year:
2004
Type:
Article
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