In vitro and In vivo Antioxidant Activity of the Total Dichloromethane-ethanol Extract of Morinda morindoides (Baker) Milne-redh. (ETDE) (Rubiaceae).

ABSTRACT

Purpose: our work consisted in studying the antioxidant activity of the dichloromethane-ethanol total extract (Baker) milne-redh., a Rubiaceae with antihypertensive activity. Methodology: The antioxidant activity was determined in vitro by the test of 2,2'-diphenyl-1- picrylhydrazyl (DPPH), the test of inhibition of lipid peroxidation by TBARS method and in vivo by determining activities of SOD, CAT and the concentration of NO in the organs of rats made hypertensive by adrenalin (ADR). In vivo, Rats were divided into 7 lots of 5 rats each. The control lot received daily orally distilled water for 14 days. The other lots received adrenalin (ADR) at dose of 1 mL/kg by intraperitoneal injection for 8 days. After installation of hypertension, lot MNT (Lot sick untreated or positive control) has not undergone any treatment. Rats of lots ETDE 500 and ETDE 1500, received orally ETDE at doses of 500 and 1500 mg/kg bw for 6 days. Rats of lots TEN (tenordate) 10 and TEN 20 received orally the tenordate at doses of 10 and 20 mg/kg pc for 6 days. After the 6 days of treatment, the rats are decapitated and their hearts, kidneys, livers andaortas were collected for the determination of oxidative stress parameters. Results: Our results showed that ETDE, in vitro, reduces greatly the DPPH with a IC50 of 21.45±1.53% and inhibits 49.66±3.83% of lipid peroxidation. In vivo, ETDE reduced significantly and normalizes the activity of SOD, CAT and normalizes the concentration of NO of rats made hypertensive. Conclusion: ETDE by its antioxidant action on free radicals may be beneficial in the treatment of high blood pressure.