Molecular Characterization of Some Virulence Factors of Streptococcus pneumoniae Isolated from Children with Acute Otitis Media in Hilla, Iraq.
Br Biotechnol J
;
2016; 10(3): 1-11
Article
in English
| IMSEAR
| ID: sea-180040
ABSTRACT
Aims:
Isolation and identification of Streptococcus pneumoniae from children with acute otitis media. Molecular detection of some virulence genes of S. pneumoniae such as pneumolysin (ply), autolysin A (LytA), Neuraminidase (NanA), Luminescence luxS, capsular polysaccharide synthesis (cpsA), pneumococcal surface antigen A (psaA), α- Enolase (Eno ) by using PCR primer. Place and Duration of Study Hilla Teaching Hospital (ENT unit) and privacy during a period from November (2014) to March (2015), Hilla, Iraq.Methodology:
A total 0f 120 Ear swabs obtained from children suffering from acute otitis media ranged from (1 months - 12 years). Only eight isolates of S. pneumoniae were isolates. Seven virulence genes were investigated in eight clinical isolates of S. pneumoniae by using PCR techniques.Results:
Pneumolysin and autolysin were seen to be present in only 4 isolates which were isolated from otitis media samples, whereas Luminescence and α - Enolase gene were present in five isolates, Besides, Neuraminidase and pneumococcal capsule synthesis A were detected in all isolates. It was found that Pneumococcal Surface antigen A is present in 2 isolates.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
Br Biotechnol J
Year:
2016
Type:
Article
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