Group A streptococcal vaccine delivery by immunization with a self-adjuvanting M protein-based lipid core peptide construct.
Article
in English
| IMSEAR
| ID: sea-18013
ABSTRACT
BACKGROUND & OBJECTIVES:
To develop a broad strain coverage GAS vaccine, several strategies have been investigated which included multi-epitope approaches as well as targeting the M protein conserved Cregion. These approaches, however, have relied on the use of adjuvants that are toxic for human application. The development of safe and effective adjuvants for human use is a key issue in the development of effective vaccines. In this study, we investigated the lipid polylysine core peptide (LCP) system as a self-adjuvanting GAS vaccine delivery approach.METHODS:
An LCP-GAS construct was synthesised incorporating multiple copies of a protective peptide epitope (J8) from the conserved carboxy terminal C-repeat region of the M protein. B10.BR mice were immunized parenterally with the LCP-J8 construct, with or without conventional adjuvant, prior to the assessment of immunogenicity and the induction of serum opsonic antibodies.RESULTS:
Our data demonstrated immunogenicity of LCP-J8 when coadministered in complete Freund's adjuvant (CFA), or administered in the absence of conventional adjuvant. In both cases, immunization led to the induction of high-titre J8 peptide-specific serum IgG antibody responses, and the induction of heterologous opsonic antibodies that did not cross-react with human heart tissue proteins. INTERPRETATION &CONCLUSION:
These data indicated the potential of a novel self-adjuvanting LCP vaccine delivery system incorporating a synthetic GAS M protein C-region peptide immunogen in the induction of broadly protective immune responses, and pointed to the potential application of this system in human vaccine development against infectious diseases.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Peptides
/
Streptococcus
/
Bacterial Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Molecular Sequence Data
/
Bacterial Vaccines
/
Blotting, Western
/
Amino Acid Sequence
/
Lipids
/
Antibodies, Bacterial
Language:
English
Year:
2004
Type:
Article
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