Crystallization Test for Early Detection of Malignancy

ABSTRACT

Background:  Cancer can involve any tissue of the body. In most of the cases, patients present themselves to a medical facility when the disease has reached an advanced stage and is not amenable to treatment. So, the line of action in order to cure cancer should be its early detection and prompt treatment of precancerous lesions. This is one of the cornerstones of cancer prevention.  Aim: Aim of the study was to determine the efficiency of crystallisation test for early detection of malignancy. Methods:  110 subjects were included in the present study. 50 subjects were normal comprising the control group and 60 subjects were diagnosed with dysplastic changes. All samples are collected by finger prick with aseptic precautions. Crystallization tests were carried out strictly maintaining all necessary conditions. The results obtained were than analysed and studied. Results: Crystallization pattern with only cupric chloride solution alone is very haphazard and completely lacking of co-ordination. The pattern of admixture of normal blood solution and cupric chloride is typical and shows co-coordinated arrangement of crystals. Blood crystallization pattern in control group shows very specific arrangement of needle like crystals of cupric chloride. Hollow Glans formation characterizes the benign condition while hollow glans along with gap star formation characterizes the precancerous conditions. Present study of malignant pattern proves that transverse bar or transverse formation is confirmatory finding in advanced cases. Conclusions: Inference of the test shows that control group had shown 100% positivity while all cases of malignant as well as premalignant conditions was shown 99% positivity.