Evaluation of Nephroprotective Effect of Ethanolic Extract of Leaves of Aloe barbadensis against Gentamicin Induced Nephrotoxicity in Rats

ABSTRACT

Background: To evaluate the nephroprotective effect of ethanolic extract of leaves of Aloe barbadensis against gentamicin induced nephrotoxicity in albino rats. Methods: In the present study 24 albino rats of 120-150gm were taken and these rats were divided into four groups. Group I and II served as vehicle control and negative control groups respectively. While group III (EEAB150) and IV (EEAB300) served as treatment groups, which were treated with 150 and 300mg/kg/day of ethanolic extract of Aloe barbadensis, 1 hour before each dose of gentamicin administration for 8 days. On the 8th day of Gentamicin administration (80mg/kg/day, i.p) blood samples for BUN, serum creatinine, urine creatinine and total protein were taken while the rat kidneys for antioxidant assay and histology were obtained. Results: In group II nephrotoxicity was confirmed by significant elevation of BUN and Serum creatinine while urine creatinine and total protein is significantly decreased compared to group I , while in treatment groups III and IV there is significant attenuation of elevated BUN and S. creatinine while urine creatinine and total protein tends to increase compared to group II. Also, there was significant changes observed in antioxidant markers in the treatment groups III and IV. Improvement in renal function was also confirmed by histology of rat kidneys. Conclusion: Ethanolic extracts of leaves of Aloe barbadensis possess nephroprotective effect against gentamicin induced nephrotoxicity.