Histological Study- The Effects Of Various Fixatives On Kidney

ABSTRACT

Fixation is the critical step in processing tissues. Following tissue removal from the body, autolysis begins and proceeds more or less quickly depending on many factors, including the level of enzymes or microorganism present in the tissue. We studied effect of five different types of fixatives. An essential part of all histological and cytological techniques is preservation of cells and tissues as they naturally occur. The aim of the current study is to see the effect of following fixatives namely 10% formalin, Buffered 10% formalin, Bouin’s fluid, Zenker’s fluid, Carnoy’s fluid on liver tissues and to observe the optimum result in a particular fixative in H&E sections. There is no single fixative which can be considered as best fixative for all purposes. Best fixatives for architectural preservation are Carnoy’s fluid and Zenker’s fluid. Best fixative for study of nuclear details is Bouin’s fluid.