Pharmacological effects of Withania somnifera root extract on GABAA receptor complex.
Article
in English
| IMSEAR
| ID: sea-18602
ABSTRACT
A methanolic extract of W. somnifera root inhibited the specific binding of [3H]GABA and [35S]TBPS, and enhanced the binding of [3H]flunitrazepam to their putative receptor sites. The extract (5 micrograms) inhibited [3H]GABA binding by 20 +/- 6 per cent whereas a concentration of 1 mg of the extract produced 100 per cent inhibition. The extract (5-100 micrograms) produced 20 +/- 4 to 91 +/- 16 per cent enhancement of [3H]flunitrazepam binding. In functional studies using 36Cl-influx assay in mammalian spinal cord neurons, W. somnifera root extract increased 36Cl-influx in the absence of GABA. This effect on 36Cl-influx was blocked by bicuculline and picrotoxin; and enhanced by diazepam. These results suggest that the W. somnifera extract contains an ingredient which has a GABA-mimetic activity.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Female
/
Pregnancy
/
Plant Extracts
/
Chlorides
/
Receptors, GABA-A
/
Gamma-Aminobutyric Acid
/
Animals
/
Mice
/
Mice, Inbred C57BL
Language:
English
Year:
1991
Type:
Article
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