Utility of XY-amelogenin gene primers for detection of sex chromosomes.
Article
in English
| IMSEAR
| ID: sea-18710
ABSTRACT
The utility of polymerase chain reaction (PCR) amplification of amelogenin gene as a reliable and rapid means of determination of sex chromosomes was tested in 20 patients of X-linked disorders (Duchenne muscular dystrophy, haemophilia and Wiscott-Aldrich and Hunter's syndromes), 12 of intersex (testicular feminization syndrome, male pseudohermaphrodites, true hermaphrodites) and 21 of congenital adrenal hyperplasia. Of these, 26 (49%) cases were for prenatal diagnosis of X-linked diseases and congenital adrenal hyperplasia (CAH). The presence of X and Y chromosomes was determined within 24 h of receiving the samples. The results were in conformity with cytogenetic studies in all instances. The analysis of amelogenin gene proved helpful in the diagnosis and management of these patients.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Prenatal Diagnosis
/
Sex Chromosome Aberrations
/
Sex Chromosomes
/
Humans
/
Child
/
Child, Preschool
/
Polymerase Chain Reaction
/
Adolescent
/
Adult
/
Dental Enamel Proteins
Type of study:
Diagnostic study
Language:
English
Year:
1998
Type:
Article
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