Using of BANA-Enzymatic™ test kit to detect periodontal heath of patients with generalised chronic periodontitis before and after scaling and root planing – A randomized control study

ABSTRACT

Background: Clinical improvements after SRP are associated with microbiological changes that include a decrease in microbial load and a mean percentage change of certain periodontal pathogens, such as Treponema denticola, Porphyromonas gingivalis and Tarnella forsythus. These species are gram negative anaerobes which possess, in vivo an enzyme capable of hydrolyzing synthetic trypsin substrate, BANA (N-Benzoyl D-L Arginine -2 Naphalamide). BANA a colorless substrate, it releases β- naphthylamide, which turns orange red when a drop of fast garnet is added to the solution. Several Bacteroides and Capnocytophaga species were occasionally BANA positive, only when in large CFU‟s. Loesche proposed the use of this BANA reaction in subgingival plaque samples to detect the presence of any of these periodontal pathogens and thus serve as a marker of disease activity. Aim and objective:The aim and objective of this study was to detect the presence of BANA microorganisms and also to determine the effect of scaling and root planning in adult periodontitis patients. Materials and Methods: 30 Subjects randomly selected comprising of both the sexes, visiting outpatient department of Periodontology, Govt. Dental College and Hospital Srinagar, were considered for the present clinical study after meeting inclusion and exclusion criteria. Subjects were randomly assigned into two groups- 15 Subjects in Control Group (Group A ) and 15 Subjects in Abhima Kumar, Prabhati Gupta, Rashida. Using of BANA-Enzymatic™ test kit to detect periodontal heath of patients with generalised chronic periodontitis before and after scaling and root planing – A randomized control study. IAIM, 2019; 6(4) 100-106. Page 101 Treatment Group (Group B ) i.e. The Control group- Group A Treatment group- Group B Four test sites were selected from each patient and assessed for plaque index, bleeding index and pocket depth and attachment loss before and after scaling and root planning. BANA test was used for the detection and prevalence of the “red complex” bacteria in plaque samples. Results: The BANA tests are statistically correlated with the severity of periodontal destruction. There was a statistically significant correlation between the BANA test results and the parameters used to test the periodontal heath. Conclusion: This study encourages the use of BANA as chair-side tests for a proper diagnosis of periodontal condition. It also gives a picture of microbiological flora of the plaque.