Analysis of various biochemical parameters of T2DM patients and healthy controls

ABSTRACT

Background: T2DM is a metabolic disease associated with a group of abnormalities including hyperglycemia, dyslipidemia, hypertension, elevated levels of biochemical and inflammatory markers in circulation. This condition predisposes an individual to a number of adverse consequences which include atherosclerotic cardiovascular disease, neuropathy, nephropathy, and retinopathy. Aim of the study To compare the various biochemical parameters in T2DM patients and healthy age-matched controls. Materials and methods: The Study included 22 type of II diabetic patients as cases and 22 normal individuals as controls. Fasting blood sugar (FBS) and Postprandial blood sugar (PPBS complete hematological profile, lipid profile, total protein, c-reactive protein and calcium levels were measured in plasma of T2DM and compare with healthy controls. Fasting blood samples were collected into labeled centrifuge tubes, after an 8–12 h overnight fast, from the subjects by venepuncture. The blood samples were centrifuged at 2000rpm for 10 min using a desktop centrifuge and the serum separated and kept in labeled sample bottles at −70°C until further analysis. Results: The results showed higher concentrations of RBC, hemoglobin, HCT, and lymphocytes in healthy controls when compared with T2DM patients and lower concentrations of WBC, platelets, MCV, MCH, neutrophils, monocytes and eosinophils in healthy controls when compared with T2DM patients. Serum lipid profiles in plasma of control and T2DM patients. The results showed high levels of serum lipid profiles including cholesterol, HDL-C, LDL-C and total cholesterol ratio in healthy age-matched controls when compared with T2DM patients. In contrast, the levels of triglycerides were found to be lower in healthy controls when compared with T2DM patients. The results showed that levels of plasma glucose, C-reactive protein, HbA1c were significantly (p<0.05) higher in T2DM Jaiprabu Jayaraj, Pandiamunian Jayabal. Analysis of various biochemical parameters of T2DM patients and healthy controls. IAIM, 2019; 6(7) 1-6. Page 2 patients when compared with those without T2DM (healthy controls). There were also significantly (p<0.01) low levels of total bilirubin, ALT, total protein, albumin, total calcium in plasma of TDM patients when compared with plasma from healthy age-matched controls. Conclusion: The findings in this study support the hypothesis that low-grade systemic inflammation is an underlying factor in the pathogenesis of T2DM and also a common antecedent for both T2DM and CVD. The data from this particular study also provide further evidence that inflammatory markers might provide a method for early detection of CVD risk. These data might have many significant implications for the prevention and treatment of T2DM. Modification of lifestyle habits and management of systemic inflammation should be the major targets for the prevention and treatment of CVD in T2DM patients