Pathological Overview and Antioxidant Status of Thyme on H2O2-induced Spleen Tissues Damage

ABSTRACT

Objectives: To study the preventive effect of Thymus algeriensis essential oil (TAS) against hydrogen peroxide (H2O2)-induced spleen toxicity in rats. Materials and Methods: Rats were treated with Hydrophobic fractions of Thymus algeriensis (180 mg/kg body weight, n=6), H2O2 (0.1, 1 mmol/L body weight, n=6) and the exposure to both drugs orally for 15 days. Histological examination was performed and the levels of biochemical parameters and lipid peroxides were determined. Results: In spleen tissue protein, catalase, superoxide dismutase, and glutathione (GST, GPx and GSH) levels were increased significantly (P<0.05) in the essential oil pretreated rats when compared to H2O2. TAS decreased the intracellular malondialdehyde (MDA) levels in spleen tissues. Vascular congestion was seen in spleen of high dose H2O2-treated rats and normal architecture of tissues was observed in other groups. Conclusion: The biochemical parameters and histopathology examination support the cytoprotective effect of Thyme which could be attributed to terpenes.