Bactericidal Effect of GaAIAs Laser on Anaerobic Photosensitized Periodontopathics- An In Vitro Study

ABSTRACT

Background: The mainstay of periodontal therapy remains the physical removal of subgingival plaque. The present study was conducted to determine bactericidal effect of GaAIAs laser on anaerobic photosensitized periodontopathics. Materials & Methods: The present study was conducted on 60 adult patients of both genders of chronic periodontitis (Group I). Equal number of controls was also selected (Group II). In all patients subgingival plaque samples were collected. Samples were cultured anaerobically for 72 h at 37°C on blood agar, Brewer's agar culture plates. Bacteria were identified based on colony characteristics, hemolysis, pigmentation, and fluorescence. Results: The mean P. gingivalis count (CFU/ml) during undilution in group I was 302.4 and in group II was 401.5, at 110 was 204.5 in group I and 328.2 in group II, at 150 was 115.2 in group I and 276.3 in group II, at 1100 was 27.8 in group I and 132.8 in group II. The difference was significant (P< 0.05). The mean F. nucleatum count (CFU/ml) during undilution in group I was 212.4 and in group II was 291.5, at 110 was 125.5 in group I and 217.2 in group II, at 150 was 68.2 in group I and 150.3 in group II, at 1100 was 17.8 in group I and 94.8 in group II. The difference was significant (P< 0.05).Conclusions: Authors found that GaAIAs laser found to be effective in reducing periodontal pathogens.