Detection of haemoglobin variants for the diagnosis of beta thalassaemia & other haemoglobinopathies using anion exchange high performance liquid chromatography.
Article
in English
| IMSEAR
| ID: sea-18996
ABSTRACT
We describe the precise identification, separation and quantitation of several haemoglobin (Hb) variants such as Hbs A2, A, F, S, E, J, H, D-Punjab and Q-India in untransfused patients with beta thalassaemia and other haemoglobinopathies prevalent in the Indian population using whole blood (5 microliters), red cell lysates (2 microliters) or dried blood specimens on filter paper, using a conventional anion exchange high performance liquid chromatography (AX-HPLC) system. Samples were simultaneously studied for the presence of haemoglobin variants by alkaline electrophoresis. The problems in analysing rare variants and separating the closely migrating variants, encountered in the conventional methods could be resolved using the HPLC technique.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Genetic Variation
/
Female
/
Humans
/
Male
/
Hemoglobins
/
Case-Control Studies
/
Child
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Beta-Thalassemia
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Language:
English
Year:
1996
Type:
Article
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