Propagation of Mycobacterium lepraemurium on supplemented minimal medium and its experimental pathogenesis.
Article
in English
| IMSEAR
| ID: sea-19131
ABSTRACT
The splenic tissue of a mouse experimentally infected with M. lepraemurium (Hawaiian strain, M-65) and developing 'rat leprosy', yielded a pure culture of an acid - fast bacterium having all the characteristics of M. lepraemurium on mineral salt minimal medium supplemented with simple sources of C and N, e.g., NH4 -salts, liquid paraffin, urea, gelatin etc. This could be maintained, by serial passages in vitro with good growth. Its indefinite propagation with tissue - free washed, small inoculum on complex media including Ogawa medium was difficult, and its serial sub-culture was practically impossible. The in vitro isolate from supplemented minimal medium could produce pathological lesions in mice typical of rat leprosy.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Culture Media
/
Animals
/
Mice
/
Minerals
/
Mycobacterium Infections
/
Mycobacterium lepraemurium
Type of study:
Etiology study
Language:
English
Year:
1995
Type:
Article
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