Evaluation of enzyme-linked immunosorbent assay in the diagnosis of kala-azar in Malda district (West Bengal).
Article
in English
| IMSEAR
| ID: sea-19282
ABSTRACT
An immunological test based on indirect (plate) ELISA has been successfully standardized and modified using promastigote soluble antigen. The test carried out on 813 subjects from a kala-azar endemic area (including parasitologically confirmed patients, subjects presenting with clinical symptoms of visceral leishmaniasis and endemic controls) and a non-endemic area (with diseases other than kala-azar and apparently normal subjects) was found to detect, specifically, antileishmanial antibodies. The plate ELISA has been simplified to a more sensitive dot-ELISA where the results are read within 2-3 h. The antigen requirement is 250 ng per test. No cross-reactivity with sera from patients of malaria, tuberculosis, leprosy, amoebiasis and filariasis was observed. The follow up monitoring of antibodies in successfully treated kala-azar patients showed a decline of antibodies. A drop of blood taken on filter paper is sufficient to conduct the test. Dot ELISA therefore is a simple, inexpensive and stable test in serodiagnosis of visceral leishmaniasis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
/
Antibodies, Protozoan
/
Leishmania infantum
/
India
/
Leishmaniasis, Visceral
/
Animals
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
English
Year:
1993
Type:
Article
Similar
MEDLINE
...
LILACS
LIS