Klingler’s Fiber Dissection Method- An Assisting Approach Towards Teaching Neuroanatomy

ABSTRACT

Background and Objective: The Klingler fiber dissection technique is a simple and less complicated method foridentifying the fine structure of the white fiber tracts of brain. In this study, we have used classical fiber dissectiontechnique by Klinger’s to produce white matter specimens which can be used for explaining anatomy of variouswhite matter tracts to students.Materials and Methods: Five brains specimen removed from formalin fixed human cadavers (3 males and 2female) were used in this study. Klinger’s fibers dissection method was used to obtain white fibers specimen.Dissection of the cerebrum was performed using wooden spatulas, fine curved metal spatulas, fine forceps. Thewhite fibers were exposed by peeling brain with help of wooden spatula to expose the fibers. The dissectionmicroscope was used to isolate small structures.Results: Using the classical Klinger’s technique, we were able to obtain a brain specimen depicting organizationof various white fibres such as corona radiata, superior longitudinal bundle, association fibres with fibrespassing in relation to lentiform nucleus. In another specimen, dissection of right cerebral hemisphere medial tolentiform nucleus showed continuity of white projection fibres of corona radiata as internal capsule. Fibres ofcorpus callosum were delineated in two specimens which displayed spatial disposition of its various parts.Conclusion: White matter fiber of brain are very important for understanding of function of the central nervoussystem function. The Klingler’s fiber dissection technique with other study material can successfully serve thepurpose of the teaching of complex brain architecture of white matter. These dissected specimens will be moreattractive to students, than the mere imagination of white fiber tracts during neuroanatomy classes.