Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus
Indian J Med Microbiol
;
2019 Mar; 37(1): 54-59
Article
| IMSEAR
| ID: sea-198837
ABSTRACT
Background:
Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens. Aim andObjectives:
A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic with periodontitis. PCR and sequencing of P. gingivalis was performed in saliva samples. Materials andMethods:
DNA was extracted from saliva using Triton X-100 and 16s rRNA gene (404 bp) was amplified by polymerase chain reaction. DNA sequencing was performed for two samples.Results:
P. gingivalis was detected in 27.03% (n = 10), of which 30% (n = 9) were diabetic with periodontal disease and 14.3% (n = 1) were diabetic without periodontal disease. The percentage of poor oral hygiene was 50% and 20% in uncontrolled and controlled glycaemic patients, respectively. DNA sequencing of two samples showed 100% identity with the sequences in the GenBank database (Gen Bank accession no KX640913-KX640914).Conclusion:
Type 2 diabetes mellitus and periodontitis are interlinked. Early detection of P. gingivalis and appropriate treatment with doxycycline will also assist in controlling the glycaemic status.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Type of study:
Observational study
/
Screening study
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2019
Type:
Article
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