Molecular confirmation of the circulating Bacillus anthracis during outbreak of anthrax in different villages of Simdega District, Jharkhand
Indian J Med Microbiol
;
2019 Mar; 37(1): 116-119
Article
| IMSEAR
| ID: sea-198849
ABSTRACT
Aims and Objectives:
Molecular confirmation of the circulating Bacillus anthracis during outbreak of anthrax in different villages of Simdega district, Jharkhand, India. Materials andMethods:
Blood samples with swabs from skin lesions (eschar) were collected from the suspected cases of Anthrax from October 2014 to June 2016 from Simdega district, Jharkhand. All the swabs were inoculated on polymyxin lysozyme EDTA thallous acetate media, nutrient agar media as well as 5% sheep blood agar media. Gamma-phage lysis was done. DNA extraction was done using a QIAamp DNA Mini Kit (QIAGEN, Valencia, CA, USA) and subjected to polymerase chain reaction (PCR) using anthrax-specific primers.Results:
On Gram and acid fast staining, purple rods and pink-coloured anthrax spores were detected. Capsular and M'Fadyean staining was done. Gamma-phage lysed B. anthracis culture. Of 39 suspected cases, 8 were culture and PCR positive and showed gamma-phage lysis. 3 deaths were reported. Discussion andConclusion:
The conventional and real-time PCR methods are suitable for both the clinical and the epidemiological practice.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2019
Type:
Article
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