Spectrophotometric assessment of effect of aqueous extract of Aloe vera on rabbit erythrocytes in varying concentrations of saline

ABSTRACT

Background: Hemolytic disorders are one of the prime reasons for frequent blood transfusions which involves lots of costs and sufferings to the patient. This study was undertaken to determine the effect of water soluble extract of Aloe vera on rabbit erythrocytes in varying concentrations of NaCl from 0.9% (isotonic) to 0.15% (hypotonic).Methods: Aqueous extract of Aloe vera (AVE) 200mg/kg was orally administered to rabbits in the test group while control group was given 1ml of distilled water (DW). Blood was withdrawn from rabbits, centrifuged and suspension in 1ml of normal saline was made. 20 microliter of red blood cells suspension from both control and test groups was added to normal saline of varying concentrations from 0.9% to 0.15% NaCl which were quantitatively analysed for hemolysis by UV spectrophotometer. Data was analysed by unpaired t test and P <0.05 was considered statistically significant.Results: The difference in percentage of hemolysis in both test and control groups was not statistically significant. Therefore, acute administration of water soluble extract of Aloe vera (200mg/kg) did not have protective effect on rabbit erythrocytes against hypotonic solution of normal saline.Conclusions: Aloe vera might be useful for the treatment of oxidative stress-related human disorders by virtue of its antioxidant activity and may have a role in prevention of hemolysis which needs to be explored by further studies.