Protective Effect of Dorema glabrum on Induced Oxidative Stress by Diazinon in Hippocampus of Rat

ABSTRACT

Introduction:Diazinon (DZN) administration produces lipid peroxidation as an indicator of oxidative stress in the brain. Some medicinal plants such as Doremaglabrumhas antioxidant properties, so can be usedas an antioxidant that may protect neurons from oxidative stress. The aim of present study was to investigate the effect of D.glabrumagainst DZN-induced oxidative stressin hippocampus.Methods:Twenty-four adult male Wistar rats were used in this study.The rats randomly were divided into four groups including a control group, and two groups received different doses of D.glabrum(40 and 80 mg/kg) as pre-treatment for 21 days with DZN (100 mg/Kg) that was injected intraperitoneally (ip) in last day of D. glabrumusage, and one group received only DZN. Thiobarbituric acid reactive substances (TBARS), which are the indicators of lipid peroxidation, and the activities of antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase) were determined in the ratsʼhippocampus.Results: Administration of DZN significantly increased TBARS levels and superoxide dismutase activity and decreased glutathione peroxidase activity but there were no significant changes in catalase activity in the hippocampus. Combined D.glabrumand DZN treatment, caused a significant increase in glutathione peroxidase, a significant decrease of TBARS and a significant decrease in superoxide dismutase and again no significant changes in catalase activity in the rats’ hippocampus when compared to the rats treated with DZN.Conclusion:Our study demonstrated that D.glabrumhad an amelioratory effect on oxidative stress induced by DZN