Effect of Combination Therapy of Garcinia kola Seed and Vitamin E in Acetaminophen Induced Hepatotoxicity and Oxidative Stress in Albino Rats

ABSTRACT

Acetaminophen, used for antipyretic and analgesic purposes has been known to exhibit toxic effects on the organs because of its ability to generate free radicals, causing varieties of diseases. This study investigated the impact of the combined formed ofGarcinia kolaseed and Vitamin E pretreatment exposure on hepatotoxicity and oxidative stress induced by acetaminophen in Albino Rats. Five groups of animals were used for this study. Group 1 as the control received distilled water orally only, group 2 astoxicity control intoxicated with 800mg acetaminophen intraperitoneally. The other three groups were pretreated with various doses of either Garcinia kolaseed extract or vitamin E or a combined form respectively by oral gavagemethod for 7 days before induction with acetaminophen intraperitoneally on the 8thday and sacrificed under chloroform anaethesia. Acetaminophen induction significantly rise (p<0.05) the hepatic enzyme levels (ALT, AST, and ALP) and a marked reduction of the antioxidant enzymes (SOD, CAT and GPX) in group 2 animals when compared with the control. There was also a significant rise (p<0.05) in the MDA levels. Meanwhile the combined form of Garcinia kolaseed extract and Vitamin E pretreatment exposure on theorgans showed no synergetic ameliorative potentials as compared with the single pretreatment exposure with Garcinia kola and Vitamin E respectively. The morphology of the tissue cells pretreated with these combined formed exhibited features showing signs of cell damages and slow recovery from the toxicity. Therefore combining Garcinia kola andVitamin E may lost its ameliorative and protective effect as seen in this induced acetaminophen albino rats implying that Garcinia kolaseed and Vitamin E should notbe taken in a combined form.