Evaluation of a modified IRMA for anti-D quantitation, using 3H protein A.
Article
in En
| IMSEAR
| ID: sea-20138
ABSTRACT
A modified immunoradiometry assay (IRMA) using tritiated (3H) protein A was developed to estimate anti-D concentration. The main advantages of the assay were longer shelf life of the labelled reagent (more than two years); minimum radiation hazard and; low non specific binding. Levels of anti-D were estimated in 23 Rh (D) immunised women. A good correlation of anti-D concentration (micrograms/ml) with Rh antibody titre was observed (r = +0.89, P < 0.01). Anti-D concentration estimated by 3H protein A IRMA correlated well with the severity of Rh-HDN. This assay could quantitate anti-D in sera having exclusively IgG3 subtype.
Full text:
1
Index:
IMSEAR
Main subject:
Rh-Hr Blood-Group System
/
Rh Isoimmunization
/
Staphylococcal Protein A
/
Tritium
/
Female
/
Humans
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Immunoradiometric Assay
/
Isoantibodies
Language:
En
Year:
1993
Type:
Article