A Comparative Analysis of Rapid Immunochromatographic Card Test with MAC –ELISA Used in Detection of Dengue at a Tertiary Care Hospital in Jamshedpur, Jharkhand

ABSTRACT

Introduction: Dengue virus transmitted by mosquitoes iswidely distributed throughout the tropics and subtropics. It isone of the most rapidly spreading mosquitos borne viraldisease in the world. In India specially, dengue has emergedas a matter of major concern because of its epidemicproportions. Therefore, early diagnosis for dengue is called for,to reduce the mortality and morbidity attributed to it.Aims and Objective: There are numerous methods for thediagnosis of dengue of which the serological tests play asignificant role. Various rapid ICT tests are being used in thelaboratories for the early diagnosis of dengue these days. Thepresent study aims to evaluate the performance of rapid ICTwith reference to MAC-ELISA for its role in diagnosis ofdengue.Methods and Materials In the present study 305 suspectedsamples of dengue were collected during the month of October2017 to December 2017. All the samples were collected byaseptic techniques.Results: 92 samples were positive by Mac Elisa IgM Ab test.The prevalence of the disease was 30.16 %. When ICT IgM Abtest was compared with Elisa IgM capture Ab test a sensitivityof 95.65 % and specificity of 98.59 % was observed.Conclusion: The high sensitivity and specificity makes itsuitable to be used in acute diagnosis of dengue but Mac ElisaIgM capture Ab test combination will make it more reliable andconfirmatory.