Influence of N-terminal amino acids & conjugation position to carrier on specificities of antibodies elicited by malaria peptides.
Article
in English
| IMSEAR
| ID: sea-20322
ABSTRACT
The specificity of murine antibodies raised against structurally related peptides derived from a malaria parasite membrane protein was studied. The peptides were conjugated to bovine serum albumin (BSA) with 6-maleimido caproic acyl N-hydroxysuccinimide ester before immunization. Conjugation to BSA through a C-terminal or an internal cysteine residue elicited antibodies with noticeably different specificities. An N-terminal tripeptide sequence arginine-asparagine-asparagine had a dominant influence on the immunogenicity of the peptides. Such factors need to be taken into consideration while designing peptide-based immunogens.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Peptide Fragments
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Protozoan
/
Molecular Sequence Data
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Protozoan Proteins
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Amino Acid Sequence
/
Animals
/
Malaria
/
Mice
/
Mice, Inbred BALB C
Language:
English
Year:
1994
Type:
Article
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