Development And Validation Of Uv-Visible Spectrophotometric Method For Determination Of Duloxetine
Int J Pharm Pharm Sci
;
2019 Mar; 11(3): 27-31
Article
| IMSEAR
| ID: sea-205851
ABSTRACT
Objective:
The aim of the present work was to develop and validate a simple UV spectroscopic method for the determination of duloxetine, which is a thiophene derivative and a selective neurotransmitter reuptake inhibitor for serotonin, norepinephrine, and to lesser degree dopamine.Methods:
The UV Spectrophotometric analysis was performed using Shimadzu UV-1800 and Shimadzu UV-1700 spectrophotometer by using solvent system acetonitrile and water in the ratio of 82. Detection was performed at a wavelength of 290 nm. Method validation was carried out according to ICH Q2R1 guidelines by taking the parameters linearity, accuracy, precision, ruggedness, and robustness, LOD and LOQ.Results:
The UV Spectrophotometric method was found linear in the range of 10-50 μg/ml. The method was rugged and robust with % relative standard deviation less than 2. The extraction recoveries were found to be higher than 99% in all experimental conditions.Conclusion:
Based upon the performance characteristics, the proposed method was found accurate, precise and rapid and suitable for the determination of Duloxetine for routine analysis.
Full text:
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Index:
IMSEAR (South-East Asia)
Journal:
Int J Pharm Pharm Sci
Year:
2019
Type:
Article
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