Analysis Of Doxorubicin And Doxorubicinol In Dried Blood Spot Of Breast Cancer Patients For Monitoring The Cardiotoxicity Of Doxorubicin
Int J Pharm Pharm Sci
;
2020 Apr; 12(4): 16-20
Article
| IMSEAR
| ID: sea-206074
ABSTRACT
Objective:
This study aimed to analyze doxorubicin and doxorubicinol levels in Dried Blood Spot (DBS) from 25 breast cancer patients who received doxorubicin in their therapeutic regiment.Methods:
DBS samples were extracted by protein precipitation method and analyzed using Ultra Performance Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS), with the Acquity UPLC BEH C18 Waters chromatography column (2.1 x 100 mm x 1.7 μm). The mobile phase consisted of 0.1% acetic acid (eluent A) and acetonitrile (eluent B) with gradient elution; the flow rate was 0.15 ml/min and runtime, 7 min. This method was linear within the concentration range of 10–200 ng/ml for doxorubicin and 4–100 ng/ml for doxorubicinol.Result:
The analysis results showed that doxorubicin levels were in the range of 11.01 ng/ml to 93.75 ng/ml and doxorubicinol was 5.80 ng/ml to 58.57 ng/ml.Conclusion:
Cumulative doses of all patients were in the range of 49.11 mg/m2to 303.70 mg/m2, which have cardiomyopathy incidence rates<4%.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Journal:
Int J Pharm Pharm Sci
Year:
2020
Type:
Article
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