Exploring factors affecting anther culture in rice (Oryza sativa L.)

ABSTRACT

Anther culture is an important in vitro culture technique for the production of double haploids. It is largely speciesand genotype specific. Asian-cultivated rice (Oryza sativa, ssp. indica) is recalcitrant to anther culture which limits itspractical application in rice breeding. Several researchers tried to optimize the medium recipes and culture techniquesfor callus induction and plantlet regeneration. Negligible response to callus induction and recovery of a higherfrequency of albino plants are the major hindrance for the use of the technique in crop improvement. Shortening theculture period and sexual hybridization of rice subgroups (japonica/indica) may be adopted to improve green plantregeneration from anther-derived callus in indica rice. However, genetic transformation technology using individualgenes related to different aspects of anther culture could be a more direct approach. Besides, the role of genotypes,physiological status of donor plant, developmental stage of pollen, pre-treatment, culture media, phytohormones, andculture conditions for successful anther culture have been discussed.