Antiproliferative activities of Althaea ludwigii L. extract on Michigan Cancer Foundation-7 breast cancer cell line

ABSTRACT

Cancer has been considered one of these major community healthiness problems. Althaea ludwigii L.(family Malvaceae)is an indigenous plant, widely distributed in Iraq. The objective of this study is to explore the antiproliferative action ofA. ludwigii L. against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and rate embryonic fibroblasts(REF) normal cell line and to identify the bioactive constituent. The aerial parts of A. ludwigii L. were grinded intofine powder then extracted with 85% methanol. Methanol extract was further partitioned with ethyl acetate. Ethylacetate fraction was chosen to test against MCF-7 by (3-[4, 5-dimethylthiazol-2-yl]-2, 5 (MTT assay) the bioactiveconstituents were investigated by high performance thin layer chromatography high-performance thin-layerchromatography (HPTLC). The data showed that ethyl acetate fraction has significant antiproliferative activity againstMCF-7 (IC50 = 35.5 µg/ml) and REF (IC50 = 1762.9 µg/ml), HPTLC showed the existence of rutin in ethyl acetatefraction. The antiproliferative activity may relate to the existence of rutin which had been approved as potent anticancer.