Phytochemical and Antimicrobial Study on the Leaf Extract of Tapinanthus dodoneifolius (Van Teigh) Loranthaceae

ABSTRACT

Leaves of Tapinanthus dodoneifoliusis used as traditional medicine for the treatment of menstrual pain, wound, gonorrhea, and control of diarrhea and dysentery which was extracted by cold maceration in methanol. The concentrated methanol extract was subsequently partitioned with petroleum ether, chloroform, ethylacetate and n-butanol. Agar wellDiffusion method was used to determine the antimicrobial activities of the extracts against Staphylococcus aureaus,Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiela pneumonia,Salmmnella typhi, Streptococcus pyogenes, Candida krusei, Candida tropicalis,andCandida albicanat different concentrations. Antimicrobial sensitivity test indicated that,the extract inhibited the growth of all microorganisms to varying degrees except Pseudomonas aeruginosaandCandida kruseirespectively. The in vitroantimicrobial screening revealed that, the extracts exhibited diverse activities against different microbe’s concentrations ranging from 2.5mg/ml-5mg/ml. The plant extract was found to contain flavonoids, terpenoids, saponins, tannins and cardiac glycosides which may be responsible for its antimicrobial properties. The result justifies the traditional use of the plant leaf in the treatment of menstrual pain, sexual transmitted diseases, wounds and control of dysentery and diarrhea Aims: This study was aimed at investigating the antihyperglycemic effect of a combined extract of Vernonia amygdalinaand croton zabensicuscompare with a hypoglycemic drug, glibenclamide.Methodology:Twenty 20 experimental animals were used (albino rats); the rats were divided equally into four groups of five rats each; namely A (control), B (glibenclamide 10mg/kg body weight), C (synergetic treatment 1000mg/kg body weight), D (synergetic treatment 500mg/kg of body weight). Diabetes was induced intraperitoneal using Alloxan Monohydrate to all the animals and their blood glucose rise above 200mg/dl.Results:It was observed that group B and group C treated with glibenclamide (10mg/kg body weight) and synergetic aqueous extract (1000mg/kg body weight) show significantdecrease in the