Coelomic fluid of Lumbricus rubellus enhances anti-prolioniferative effect of 5-fluorouracil by modulating focal adhesion kinase express and IL-1β of colorectal cancer in mice

ABSTRACT

The different malignancy advancing systems related with focal adhesion kinase (FAK) can be attentive in theprogression of colorectal cancer. By inhibiting the growth mechanism of mixture of 5-Fluorouracil (5-FU) andearthworm therapy could enhance the affectability of malignant growth cells. The objective of this research was toexplore the antiproliferative activity of mixed coelomic fluid cancer medicines produced from Lumbricus rubellus.Colorectal cancer was actuated in mice by injected HT-29 cells in the caecum of mice, and then 5% dextran sodiumsulfate were given by the drinking water. To investigate the effect of combination treatment, we divided of fivetreatment groups group 1 was untreated (vehicle), groups 2–5 were given by 5-FU. Groups 3–5 were given by acombination of 5-FU (50 μg/g BW) and coelomic fluid (50, 100, and 200 μg/g BW), respectively. The administratedof coelomic fluid was started from the 4th weeks. Mice were sacrificed at the end of 8 weeks and colon tissue wasisolated, then assessed using Flowcytometry and immunofluorescent. These findings revealed that the mixture of 5FUand coelomic fluid components inhibits growth considerably, Interleukin-1β (IL-1β) and FAK compare with 5-FUonly group (p < 0.001; p < 0.05; and p < 0.001). Treatment used coelomic fluid at a dose of 200 μg/g BW decreasedFAK expression. The combination of 5-FU and coelomic fluid at a dose of 200 μg/g BW decreased the percentage ofIL-1β (p < 0.05).