Optimization of camptothecin production and biomass yield from endophytic fungus Fusarium solani strain ATLOY-8

ABSTRACT

Fusarium solani strain ATLOY-8, a fungus that produces camptothecin (CPT) was isolated from Chonemorphafragrans (Moon) Alston. The process of optimization of the camptothecin yield was optimized by means of thetraditional methods and analytical tools. Using the Box–Behnken design (BBD) matrix at N = 17, three independentvariables were optimized designated using one factor at a time (OFAT) method, for improved CPT and biomassproduction. The BBD exhibited 1.4- and 1.2-fold increase of CPT production and biomass yield, respectively,compared to OFAT approach in the basal medium containing absolute ethanol (5%), glucose (1%), and precursors (acombination of tryptophan, geraniol, and tryptamine; 0.03%). Analysis of variance showed the elevated coefficient ofdetermination (R2) of 0.9751 and 0.9980, respectively, for CPT and biomass yielding at a significant level (p > 0.05).Three dimensional graphs revealed dependent synergy between two variables to improve the CPT and biomass yield