A validated LC-MS/MS method for the pharmacokinetic study of alogliptin in healthy rabbits

ABSTRACT

A liquid chromatography-tandem mass spectrophotometric (LC-MS/MS) method was developed for quantification ofAlogliptin in rabbit plasma employing Liquid-Liquid extraction technique. The developed method was validated forspecificity, precision, accuracy, recovery, and stability characteristics. Chromatographic separation was achieved onInertsil ODS 5 µm C18, 50 × 4.60 mm with 3070 v/v of 0.1% formic acids Organic Mixture (acetonitrile methanol,8020% v/v) as an isocratic mobile phase with a flow rate of 1.0 ml/min. the developed LC-MS method was appliedto assess pharmacokinetics parameters of alogliptin tablet in healthy rabbits. Alogliptin showed Tmax of 2.6 ± 0.37 hand mean Cmax, AUC 0-t and AUC0 for test formulation is 90.6 ± 4.41, 1675.41 ± 164 and 3726.47 ± 796 respectively