Restoration of pro-inflammatory cytokines and histopathological changes in pancreas and liver of hyperglycemic rats by Murraya koenigii leaves extract

ABSTRACT

This study aims to investigate whether the characteristic of anti-inflammatory and protective effect of hepato- pancreascould mediate the antihyperglycemic effect of Murraya koenigii. Twenty-five Sprague-Dawley rats were inducedhyperglycemia by streptozotocin and nicotinamide (STZ-NA). The hyperglycemic rats were treated with an ethanolicextract of M. koenigii 200 mg/kg b.w and 400 mg/kg b.w. One group of the rats was treated with glibenclamide (1 mg/kg b.w). After M. koenigii extract and glibenclamide for 4 weeks, the rats were sacrificed. Blood and organ sampleswere collected under a fasting condition. The evaluation was made towards the expression of pro-inflammatorycytokines (TNF-α, IL-1β) as well as the histopathological change in the liver and pancreas. The beneficial effects ofMurraya ko enigii would bring the anti-inflammatory and hepatoprotective effect and increased the number and sizeof islet Langerhans. This research reveals that M. koenigii has a charateristic of anti-inflammatory, hepatoprotective,and it contributes to regeneration of damaged pancreatic islet. This effect is accompanied by a decrease in plasmaglucose levels