Oxyresveratrol inhibits cellular tyrosinase-related oxidative stress-induced melanogenesis in B16 melanoma cells

ABSTRACT

Cellular oxidative stress is caused by an imbalance in the redox status and manifests as hyperpigmentation disorders.Reactive oxygen species, particularly hydrogen peroxide (H2O2) as the highly reactive hydroxyl radicals, promotethe melanin production through the induction of tyrosinase enzyme activity. In this study, the antioxidant activity ofoxyresveratrol was investigated by 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. In addition, melanin biosynthesis, tyrosinase activity, and cellular oxidants due to thebioactive component, oxyresveratrol, were determined in B16 cells by melanin content assay, cellular tyrosinase activityassay, and the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay, respectively. Hydrogen peroxide induced themelanogenesis through tyrosinase activity-related cellular oxidants, whereas oxyresveratrol showed a potent antioxidantactivity by DPPH and ABTS assays. At the concentrations of 10 and 12.5 µg/ml, oxyresveratrol significantly inhibitedmelanogenesis in B16 melanoma cells and also suppressed tyrosinase activity and cellular oxidants. Effective doses ofoxyresveratrol inhibit melanogenesis through bioactivity of cellular tyrosinase-related oxidative stress