Hematological Alterations Induced by Imidacloprid and Ameliorative Effect of Withania somnifera in Female Albino Wistar Rats

ABSTRACT

Present study was aimed to evaluate the effect of oral administration of imidacloprid on weekly body weights and hematological parameters in female rats and also to determine the protective role of Withania somnifera against imidacloprid induced toxicity. Forty eight (48) female albino Wistar rats were divided into four (4) groups of twelve (12) animals each. Group 1 served as control, groups 2 was given with imidacloprid at the rate of 30 mg/kg b.wt/day, group 3 was maintained as Withania somnifera (WS) control (1g/ kg feed) and group 4 was treated with both imidacloprid + Withania somnifera (dose as above). The experiment was carried out for a period of 30 days and the test compound was administered daily by oral gavage. Blood samples were collected on 15th and 30th day for hematological analysis. A significant (P < 0.05) reduction in weekly body weights were observed in group 2. Hematology revealed a significant (P < 0.05) decrease in TEC, Hb, PCV, MCV, MCH and MCHC and increase (P < 0.05) in TLC in group 2. The DLC revealed a significant (P < 0.05) increase in neutrophil count and significant (P < 0.05) decrease in lymphocyte count in group 2. Administration of Withania somnifera along with imidacloprid brought moderate protection in all the above parameters