Isolation of pathogenic microorganisms from burn patient and in vitro determination of antibacterial activity of honey against antibiotic resistance isolates

ABSTRACT

Background:Honey is a natural therapeutic agent which manifest antimicrobial activity against a wide range of bacteria. Therefore, the current study was designed to isolate pathogenic bacteria from burn wound and also to determine the anti-bacterial traits of natural and processed honey against infectious agents.Methods:Wound samples were collected from burn unit of Dhaka Medical College Hospital and conventional cultural methods were applied to identify pathogenic microorganisms. A total of six samples including three each of natural and processed honey were tested for the determination of antimicrobial activity by agar well diffusion method.Results:Among ten wound samples highest load of total viable bacteria was recorded up to 3.7×106cfu/ml. The maximum load of Pseudomonas spp.and Staphylococcus spp.were found up to 1.6×104cfu/ml and 8.7×104cfu/ml respectively. Significant in vitroantimicrobial activity was found in all the samples. Natural honey showed a little bit more efficacy than processed honey. The samples exhibited antibacterial traits against Staphylococcus aureuswith a wide zone of inhibition and moderate zone of inhibition against Pseudomonas spp.when they are subjected to 100% concentered honey. E. coliand Klebsiella spp.were remained to be unaffected at 75% and 50% concentrated honey, while S. aureusand Pseudomonas spp.werefound to be sensitive at those concentrations.Conclusions:The in vitroefficacy of different types of honey tested against the bacteria dependent on the type of honey and the concentration at which it was administered. In our study 100% concentred honeywas more efficient in inhibiting all the tested isolates